Irvine, Calif., Nov. 3, 2016 — Few treatments exist for bacteria-caused intestinal inflammation that leads to diarrhea, fever and abdominal cramps. But University of California, Irvine microbiologists have demonstrated a new approach that may lead to more effective remedies.
In the journal Nature, Manuela Raffatellu, associate professor of microbiology and molecular genetics, and colleagues provide the first evidence that small protein molecules called microcins, produced by beneficial gut microbes, play a critical part in blocking certain illness-causing bacteria in inflamed intestines.
In their study, the researchers show that a probiotic strain of E. coli called Nissle 1917 utilizes microcins to inhibit the pathogen salmonella and an invasive form of E. coli (isolated from patients with inflammatory bowel disease).
"Although an in vivo role for microcins has been suggested for 40 years, it has never been convincingly demonstrated,” said Raffatellu, who is affiliated with UC Irvine’s Institute for Immunology.
"We hypothesize that their role was missed because, as our data indicate, microcins do not seem effective in noninflamed intestines. In contrast, we show that in an inflamed intestine, microcins help a probiotic strain limit the growth of some harmful bacteria."
She added that microcins are essential for the therapeutic activity of E. coli Nissle, and her next step is to purify microcins and test whether they can be given as targeted antibiotics.
Martina Sassone-Corsi, Sean-Paul Nuccio, Henry Liu, Dulcemaria Hernandez, Christine Vu, Amy Takahashi and Robert Edwards of UC Irvine contributed to the study, which is abstracted at http://www.nature.com/nature/journal/vaap/ncurrent/full/nature20557.html (subscription needed for complete paper).
It was supported by National Institutes of Health grants AI083663, AI101784, AI114625 and AI105374, as well as the Burroughs Wellcome Fund.